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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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Data corrente: |
09/10/2013 |
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Data da última atualização: |
09/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, A. A.; ARAGÃO, C. W. S.; CASTRO, M. E. B. de; OLIVEIRA, J. V. de C.; SOSA-GÓMEZ, D. R.; RIBEIRO, B. M. |
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Afiliação: |
ANABELE AZEVEDO LIMA, UNB; CLARA WANDENKOLCK SILVA ARAGÃO, UNB; MARIA ELITA BATISTA DE CASTRO, CENARGEN; JULIANA VELASCO DE CASTRO OLIVEIRA, USP; DANIEL RICARDO SOSA GOMEZ, CNPSO; BERGMANN MORAIS RIBEIRO, UNB. |
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Título: |
A recombinant Anticarsia gemmatalis MNPV Harboring chiA and v-cath genes from Choristoneura fumiferana defective NPV induce host liquefaction and increased insecticidal activity. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
PLOSone, v. 8, n. 9, e74592, Sept. 2013. |
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DOI: |
10.1371/journal.pone.0074592 |
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Idioma: |
Inglês |
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Conteúdo: |
One of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed higher chitinase and cysteine protease activities in insect cells and insects infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. The introduction of chiA and v-cath genes into the genome of AgMNPV improves its insecticidal activity against A. gemmatalis larvae and this recombinant virus could be used as an alternative to the wild type virus to control this important insect pest. MenosOne of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed hi... Mostrar Tudo |
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Thesagro: |
Soja. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/90688/1/journal.pone.0074592.pdf
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Marc: |
LEADER 02600naa a2200205 a 4500
001 1976569
005 2023-03-09
008 2013 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0074592$2DOI
100 1 $aLIMA, A. A.
245 $aA recombinant Anticarsia gemmatalis MNPV Harboring chiA and v-cath genes from Choristoneura fumiferana defective NPV induce host liquefaction and increased insecticidal activity.$h[electronic resource]
260 $c2013
520 $aOne of the interesting features of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D) genome is the absence of chitinase (chiA) and cathepsin (v-cath) genes. This characteristic may be responsible for the lack of liquefaction and melanization in A. gemmatalis larvae killed by AgMNPV-2D infection. This study aimed to test the hypothesis that CHIA and V-CATH proteins from Choristonera fumiferana DEF multiple nucleopolyhedrovirus (CfDEFNPV) are able to liquefy and melanize the cuticle of A. gemmatalis larvae infected by a recombinant AgMNPV containing chiA and vcath genes inserted in its genome. A fragment from the CfDefNPV genome containing chiA and v-cath genes was inserted into the genome of AgMNPV-2D. The recombinant virus (vAgp2100Cf.chiA/v-cath) was purified and used to infect insect cells and larvae. Transcripts of v-cath and chiA genes were detected along the infection of insect cells by qRT-PCR, from early to late phases of infection. The analysis of A. gemmatalis larvae killed by vAgp2100Cf.chiA/v-cath infection confirmed the hypothesis proposed. The vAgp2100Cf.chiA/v-cath showed higher insecticidal activity against third instar A. gemmatalis larvae when compared to AgMNPV-2D. The mean time to death was also lower for the vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D at 10 days post infection. Occlusion body production was higher in A. gemmatalis larvae infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. Enzyme assays showed higher chitinase and cysteine protease activities in insect cells and insects infected with vAgp2100Cf.chiA/v-cath when compared to AgMNPV-2D. The introduction of chiA and v-cath genes into the genome of AgMNPV improves its insecticidal activity against A. gemmatalis larvae and this recombinant virus could be used as an alternative to the wild type virus to control this important insect pest.
650 $aSoja
700 1 $aARAGÃO, C. W. S.
700 1 $aCASTRO, M. E. B. de
700 1 $aOLIVEIRA, J. V. de C.
700 1 $aSOSA-GÓMEZ, D. R.
700 1 $aRIBEIRO, B. M.
773 $tPLOSone$gv. 8, n. 9, e74592, Sept. 2013.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| 128. | | MOREIRA, A.; FAGERIA, N. K.; SOUZA, G. B. de; FREITAS, A. R. de. Yield, nutritional status and soil chemical properties as response to cattle manure, reactive natural rock phosphate and biotite schist in Massai grass. In: SYMPOSIUM OF THE INTERNATIONAL SCIENTIFIC CENTER OF FERTILIZAR, 18., 2009, Abstract...Rome: CRA, 2009. p. 2-6. p. 2-6| Tipo: Artigo em Anais de Congresso |
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| 130. | | DEL SANTO, V. R.; SOUZA, G. B. de; NOGUEIRA, A. R. de A.; PICCHI, C. M. C.; GARCIA, C. H. Calibração multivariada para determinação de componentes de forrageiras por meio de espectroscopia de infravermelho próximo (NIRS). In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010.| Tipo: Resumo em Anais de Congresso |
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| 133. | | BIANCHI, S. R.; NOGUEIRA, A. R. de A.; MOREIRA, A.; SOUZA, G. B. de; MENEZES, E. A.; LOPES, W. V. Alterações nos atributos químicos de um latossolo vermelho amarelo distrófico com adição de vinhaça. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: anais. Londrina: Embrapa Soja: SBCS: IAPAR: UEL, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
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| 134. | | DIAS, M.; DEL SANTO, V. R.; FERREIRA, A.; SOUZA, G. B. de; NOGUEIRA, A. R. de A. Avaliação do comportamento do espectro no infravermelho próximo de amostras submetidas à irradiação gama. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 6., 2014, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2014. p. 93| Tipo: Resumo em Anais de Congresso |
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